Construction of pcdna3.i
WebMethods: A bicistronic vector pcDNA3.0BA was constructed from pcDNA3.0. HCV PC154 gene and HBV preS2S gene were inserted into this vector to form bicistronic expression construct pcDNA3.0BAPC154S2S and monocistronic expression construct pcDNA3.0BAPC154 or pcDNA3.0BAS2S. WebNov 19, 2024 · Plasmid construction. pcDNA3.1 plasmids encoding wide type β-catenin and FLAG-MYO18A were purchased from Tsingke and GenePharma Company. S675A mutation of β-catenin and MYO18A-5A mutation were ...
Construction of pcdna3.i
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WebOct 26, 2024 · The pcDNA3.1 vector is a plasmid commonly used in recombinant protein expression in mammalian cells. This plasmid is also widely used to develop recombinant DNA-based vaccines for infectious diseases and other purposes, such as cancer gene therapy [ 15, 16, 17, 18 ]. WebMar 14, 2024 · We provide a detailed protocol on how to design and construct a custom donor vector using Gibson assembly (and the commonly utilized pcDNA3 vector as the backbone) allowing researchers to obtain specific gene modifications of interest (gene truncation, gene deletion, epitope tagging or knock-in mutation).
WebContents: First plasmid construction：pCDNA3.0-PGK-BSD Annotation：pCDNA3.0 is a backbone plasmid ideal for transient expression in our designated cell lines including HEK293T,HepG2 and Bocs. All elements of our project are first to be incised into pCDNA3.0 and tested during transient expression in cell lines. WebDec 31, 2024 · Furthermore, pcDNA3 expressing EGFP was used as a control for background level signaling and for evaluating transfection efficiency, except for bioplex experiments, for which pcDNA3 alone was used. The integrity of all constructs was confirmed by sequencing. The extracellular chemokine domain of human fractalkine was …
WebJun 15, 2015 · Construction of recombinant pcDNA3-HBsAg-p30-ROP Fresh recombinant plasmid pcDNA3-p30-ROP2 was extracted. Restriction enzyme digestion was performed … WebJul 14, 2024 · pcDNA3.1 (+) and pcDNA3.1 (-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. The high-level stable and non-replicative transient expression can be carried out in …
WebJul 1, 2015 · pcDNA3.1 (+) (Invitrogen, Groningen, the Netherlands) was used as vector for p construction, unless otherwise indicated. Murine pCCL19 was described previously . Truncated gB (730t; pgB) was amplified from the HSV-2 genome (G strain; LGC Standards), as described previously , and subcloned into pcDNA3.1 (+) . Truncated gB also was …
WebThe pcDNA3.1+/mtb32C plasmid was transformed into E. coli JM109 and then extracted. The mpt51 gene and pcDNA3.1+/mtb32C plasmid were both digested with EcoRI and BamHI restriction enzymes... ge jt3800shss manualWebWe present a detailed method for constructing a mammalian cell-based full-length antibody display library for targeting hepatocellular carcinoma. Two novel mammalian library vectors pcDNA3-CHm and pcDNA3-CLm were constructed that contained restriction enzyme sites NheI, ClaI and antibody constant domain. dda architect vacancyWebMar 8, 2024 · Plasmid construction. pcDNA3.1 (+) vector was purchased from Thermo Fisher Scientific. STXBP5-AS1 DNA was obtained by PCR using cDNA, Pfu DNA polymerase and synthetic oligonucleotide primers incorporating restriction sites. PCR products were ligated into the pcDNA3.1 (+) vector according to the manufacturer’s … dd Aaron\u0027s-beardWebHence, a DNA vaccine, named pCDNA3.1 (+)-MCP-Flag, was constructed by inserting the cloned LMBV major capsid protein (MCP) gene into the pCDNA3.1 (+)-Flag plasmid. The expression of the recombinant plasmid was confirmed by Western blot (WB) and RT-PCR. dd Aaron\\u0027s-beardWebMar 14, 2024 · We provide a detailed, step-by-step protocol for donor vector design and construction using the pcDNA3 vector . Custom donor vectors can be difficult to clone and expensive to purchase. We provide a simple, efficient protocol to obtain custom donor vectors from the common pcDNA3 mammalian expression vector. gej thickeningWebJul 1, 2016 · pcDNA3.1-e green fluorescent protein (GFP) is an important compound that is already established and widely used as a marker in biomolecular works. Producing pcDNA3.1-eGFP is not very complicated. ge jtp75sp1ss spec pdfWebApr 10, 2024 · In addition, the pcDNA3.1(+)/CPSIT_p7 vaccine diminished pulmonary pathological lesions and reduced the C. psittaci load in the lungs of infected mice. It is worth noting that pcDNA3.1(+)/CPSIT_p7 suppressed C. psittaci dissemination in BALB/c mice. ... Briefly, the construction method of pcDNA3.1(+)/CPSIT_p7 plasmid was that the target … dda athletics